Bovine hydroxyindole-O-methyltransferase. Significant sequence revision.
نویسندگان
چکیده
منابع مشابه
Molecular cloning and nucleotide sequence of cDNA encoding hydroxyindole O-methyltransferase of bovine pineal glands.
Messenger RNA for hydroxyindole O-methyltransferase (EC 2.1.1.4) was partially purified from poly(A)+ RNA isolated from bovine pineal glands by sucrose density gradient centrifugation. The enriched mRNA was used to prepare a cDNA library by use of expression vector lambda gt11. The library was screened with monoclonal antibodies to the enzyme, and three cDNA clones were isolated. These cloned c...
متن کاملLocalization of hydroxyindole-O-methyltransferase in the mammalian pineal gland and retina.
The pineal hormone, melatonin, has been reported to be synthesized in the retina by the enzyme, hydroxyindole-O-methyltransferase (HIOMT). Several laboratories have suggested that melatonin may be involved in photoreceptor outer segment disc shedding, photomechanical movements, and neuromodulation, but the cellular location of the retinal synthesizing enzymes has not been determined yet. Antise...
متن کاملSome properties of pineal gland hydroxyindole-O-methyltransferase from black rhinoceros (Diceros bicornis).
Pineal glands were obtained from two young female black rhinoceri that had died as a result of postcapture trauma during a translocation exercise. Hydroxyindole-O-methyltransferase (HIOMT) from these pineal glands showed a peak activity at pH 8.2, although high activity extended over a fairly wide pH range (7.8-8.4). N-acetylserotonin was the best hydroxyindolic substrate for the enzyme, althou...
متن کاملControl of Hydroxyindole O-methyltransferase Activity in the Rat Pineal Gland by Environmental Lighting.
The mammalian plineal rland has been shown to contain several enzymes involved in the formation and metabolism of physiologically active indoles and amines (1-3). The rat plineal (lan(l contains the highest rel)orted tissue levels of serotonin and 5-hvdroxytryptophan decarboxylase (4, 5). In addition, the plineal gland is unique in that it is the only organ that contai ns hydloxvindole O-methvl...
متن کاملInhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase.
Lactoferrin (LF) is a well-known multifunctional protein. In this study, we report the inhibitory potency of bovine LF (bLF) on catechol-O-methyltransferase (COMT), which catalyzes methylation of catechol substrates. We found that bLF binds to and inhibits COMT using its N-terminal region. An N-terminal peptide fragment obtained from bLF by trypsin digestion showed a higher inhibitory activity ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)42748-2